Wu grew up in Liuhe, a small town near Shanghai. Her father opened the first girls' school there. He believed education should not discriminate by gender. Wu attended her father's school. She learned mathematics, science, and politics. She read newspapers and debated current events with her father's friends. This was unusual for a girl in 1920s China.
At age 11, Wu left home for boarding school in Suzhou. The school had a teacher-training program. Wu studied science and mathematics. She graduated in 1929 and considered becoming a teacher. But she wanted more education. In 1930, Wu enrolled at National Central University in Nanjing. She chose physics. Few women studied physics in China. Wu did not let this deter her.
At university, Wu joined student protests against Japanese aggression. She participated in demonstrations demanding government action. She balanced activism with academics. She graduated in 1934 at the top of her class. She stayed at the university to teach and research. She published papers on X-ray crystallography. But opportunities in China were limited. She decided to study abroad.
Wu applied to the University of Michigan for graduate school. She won a scholarship. She sailed to San Francisco in August 1936. She planned to visit Berkeley before heading to Michigan. At Berkeley, she met scientists working on the cyclotron. She learned about cutting-edge nuclear physics. She decided Berkeley offered better opportunities. She changed her plans and enrolled at Berkeley. It was the right choice. She earned her PhD in 1940 under Emilio Segrè.